Mnemonic

Blood Film Abnormalities

A memory aid for the diagnostic features on a blood film.

Expansion

Each morphological abnormality points to a specific diagnosis

Mnemonic

Each morphological finding points to a diagnosis:

  • Target cells - liver disease, thalassaemia, iron deficiency, hyposplenism
  • Howell-Jolly bodies - hyposplenism, nuclear remnants normally removed by the spleen
  • Spherocytes - hereditary spherocytosis or autoimmune haemolysis
  • Schistocytes (fragments) - microangiopathy: DIC, TTP, HUS, mechanical valves
  • Sickle cells - sickle cell disease
  • Pencil cells - iron deficiency
  • Basophilic stippling - lead poisoning, thalassaemia, sideroblastic anaemia
  • Heinz bodies and bite cells - G6PD deficiency
  • Hypersegmented neutrophils - B12 or folate deficiency
  • Rouleaux - myeloma, raised ESR
  • Smear (smudge) cells - chronic lymphocytic leukaemia
  • Auer rods - acute myeloid leukaemia
  • Tear drop cells - myelofibrosis

“Howell-Jolly means no spleen; schistocytes mean something is shredding the cells; hypersegmented means megaloblastic.” Those three carry most of the clinical weight.

Expansion

Finding Suggests
Schistocytes (fragments) Microangiopathic haemolysis: DIC, TTP, HUS; prosthetic valve
Spherocytes Hereditary spherocytosis, autoimmune haemolysis
Target cells Liver disease, thalassaemia, iron deficiency, hyposplenism
Howell-Jolly bodies Hyposplenism or splenectomy
Heinz bodies and bite cells G6PD deficiency
Basophilic stippling Lead poisoning, thalassaemia, sideroblastic anaemia
Hypersegmented neutrophils B12 or folate deficiency
Rouleaux Myeloma, high ESR states
Teardrop cells Myelofibrosis, marrow infiltration
Smudge cells CLL
Auer rods AML
Sickle cells Sickle cell disease
Leucoerythroblastic film Marrow infiltration or fibrosis

The leucoerythroblastic picture (nucleated red cells and immature white cells together) is worth singling out: it means the marrow architecture is disrupted, by malignant infiltration, myelofibrosis or severe sepsis, and always warrants investigation.

Blister and bite cells with Heinz bodies after an oxidative stress in a male of African or Mediterranean origin is essentially diagnostic of G6PD deficiency.