Mnemonic

Gene Regulation

A memory aid for the levels at which gene expression is controlled.

Expansion

Transcriptional, post-transcriptional, translational and post-translational

Expansion

Transcriptional (the principal level)

  • Promoters and enhancers bound by transcription factors
  • Chromatin structure: DNA wrapped tightly on histones is inaccessible
  • Epigenetic modification: DNA methylation at CpG islands silences genes; histone acetylation opens chromatin and activates them

Post-transcriptional: alternative splicing, allowing one gene to make many proteins; RNA stability; microRNA silencing.

Translational: initiation factor availability, iron response elements.

Post-translational: phosphorylation, glycosylation, cleavage, and targeted degradation by the ubiquitin-proteasome system.

Epigenetics in medicine: genomic imprinting explains why Prader-Willi and Angelman syndromes arise from the same chromosomal region depending on the parent of origin; X inactivation explains mosaicism in female carriers of X-linked disease; and hypermethylation silencing tumour suppressor genes is a common step in carcinogenesis, which is the rationale for demethylating agents in myelodysplasia.