Mnemonic

Cystic Fibrosis Biochemistry

A memory aid for the molecular defect in cystic fibrosis.

Expansion

A defective chloride channel causes thick secretions in every exocrine organ

Expansion

Defect: the CFTR gene on chromosome 7 encodes a cyclic AMP-regulated chloride channel. Autosomal recessive; the commonest mutation is delta F508, a deletion of three bases removing a single phenylalanine.

Consequence: impaired chloride and therefore water secretion, so exocrine secretions are thick and dehydrated throughout the body.

Organ Consequence
Lung Impaired mucociliary clearance, chronic infection with Pseudomonas and Staphylococcus, bronchiectasis
Pancreas Exocrine insufficiency with steatorrhoea; later CF-related diabetes
Gut Meconium ileus in the neonate, distal intestinal obstruction later
Liver Biliary cirrhosis
Sweat glands High sweat chloride, the basis of the diagnostic test
Reproductive Absent vas deferens in males, causing infertility

The sweat test is diagnostic because the defect works in the opposite direction there: the duct normally reabsorbs chloride, so a faulty channel leaves sweat salty.

Modulator drugs are now transforming treatment by targeting the protein itself: correctors improve trafficking of misfolded delta F508 protein, and potentiators improve channel opening.